| Group | Club/Team Name | Team ID | Head Coach |
| A1 | FC Brighton FC Brighton 07B Red |
CS31-31CB16-0016 | Juan Hinojos |
| A2 | Colorado Rush Colorado Rush - 2007B Nero |
CS24-24CB16-0069 | Dewayne Edwards |
| A3 | Real Colorado RC '07B Invictus |
CS53-53CB16-0210 | J Doehring |
| A4 | Real Colorado Real CO 2007 Boys Red |
CS53-53CB16-0218 | Oran Smith |
| B1 | Broomfield Soccer Club Broomfield Blast 2007 Boys Academy |
CS08-08CB16-0098 | Chris Ellis |
| B2 | Colorado Rapids Colorado Rapids Castle Rock 2007B Select |
CS23-23CB16-0449 | Zach Ashland |
| B3 | Pueblo Rangers SC Pueblo Rangers SC 2007 Boys Elite |
CS52-52CB16-0002 | Jason Miller |
| B4 | B-Elite Soccer Academy of Colorado Springs B-Elite COS 07 Academy Boys - BU16 |
CS04-04CB16-0017 | Marcus Corbett |
| C1 | Boulder County United Boulder County United 07 Boys Academy Gold |
CS06-06CB16-0081 | Brad Homeyer |
| C2 | Colorado EDGE Soccer Club Colorado EDGE United 07B |
CS54-54CB16-0087 | Balen Arenas |
| C3 | North Denver Rush North Denver Rush B 2007 Academy |
CS44-44CB16-0015 | Michael Hill |
| C4 | Real Colorado Real CO 2007 Boys Olympico |
CS53-53CB16-0217 | Alfred Garza |
| D1 | Colorado United Colorado United - 2007B White |
CS25-25CB16-0112 | Daniel Woolliscroft |
| D2 | Pride SC Pride SC Copa '07B |
CS51-51CB16-0094 | Haillie Patterson |
| D3 | B-Elite Soccer Academy of Denver B-Elite DEN 07B Premier |
CS05-05CB16-0029 | Varol Tuncay |
| D4 | Broomfield Soccer Club Broomfield Blast 2007 Boys Prestige |
CS08-08CB16-0099 | Timothy Allen |
| E1 | Boulder County United Boulder County United 07 Boys Academy Silver |
CS06-06CB16-0082 | Jack Perkins |
| E2 | Colorado Rapids Colorado Rapids Central 2007B Select II |
CS23-23CB16-0444 | Alex Agne |
| E3 | Arsenal Colorado Arsenal Colorado 2007B Gold |
CS01-01CB16-0067 | Eric Fanning |
| E4 | Colorado Rapids Colorado Rapids North 07B Select II |
CS23-23CB16-0259 | Mike Rosso |