| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Colorado Rapids Colorado Rapids North 08B Select I |
CS23-23CB16-0254 | Temo Gonzalez-Montoya |
| A2 | Real Colorado Real CO 2008B Red |
CS53-53CB16-0215 | Eric Quintana |
| A3 | Vail Valley SC VVSC U16 Boys Navy |
CS70-70CB17-0024 | Joel Rabinowitz |
| A4 | Chivas Denver Soccer Academy Chivas Denver - BU16 AJR |
CS14-14CB16-0027 | Ismael Jr Rodriguez Hernandez |
| B1 | Colorado Rapids Colorado Rapids South 08B Select I |
CS23-23CB16-0684 | Dmytro Chepura |
| B2 | Real Colorado Real CO 2008B Olympico |
CS53-53CB16-0214 | Alfred Garza |
| B3 | Broomfield Soccer Club Broomfield SC - 2008 Boys Academy |
CS08-08CB16-0130 | Lawrie O'Toole |
| B4 | Colorado EDGE Soccer Club Colorado EDGE 2008 Boys United-U16 |
CS54-54CB16-0197 | Jeffrey Prakhine |
| C1 | Real Colorado Real CO 2008B North Athletico |
CS53-53CB16-0209 | Joseph Velotta |
| C2 | Real Colorado Real CO 2008B North National |
CS53-53CB16-0208 | Brian Cvilikas |
| C3 | B-Elite Soccer Academy of Denver B-Elite DEN 08B Academy |
CS05-05CB16-0017 | Alexis Amador Gutierrez |
| C4 | Colorado International SA CISA Academy - BU16 |
CS21-21CB16-0018 | Luis Belman Romero |