| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Colorado EDGE Soccer Club Colorado EDGE 2008 Boys Legends-U16 |
CS54-54CB16-0198 | Kyle Paine |
| A2 | Boulder County United Boulder County United 08 Boys Advanced Gold |
CS06-06CB16-0077 | Sterling Liska |
| A3 | Chivas Denver Soccer Academy Chivas Denver - BU16 BJR |
CS14-14CB16-0026 | Ismael Jr Rodriguez Hernandez |
| A4 | Colorado Rapids Colorado Rapids Central 08B Select II B |
CS23-23CB16-8508 | n/a |
| A5 | Colorado Rapids Colorado Rapids South 08B Burgundy |
CS23-23CB16-0685 | Logan Stapleton |
| A6 | Colorado Rush North Denver Rush - 2008B Nero |
CS24-24CB16-0014 | Joel Whalen |
| A7 | Boulder County United Boulder County United 08 Boys Advanced Silver |
CS06-06CB16-0078 | Miguel Prieto |
| A8 | FC Brighton FC Brighton 08B White |
CS31-31CB16-0017 | Stephen Federocko |
| A9 | Northern Colorado Rush NorCo Rush 08B Blue (Colin) |
CS45-45CB16-0043 | Mario Colin |
| A10 | Pride SC Pride SC White B08 |
CS51-51CB16-0143 | Caleb Kramer |
| A11 | Skyline Skyline 08B White |
CS62-62CB16-0171 | Dorgham Alkabi |
| A12 | Alliance Soccer Club Alliance Soccer Club - BU16 |
CS77-01CB16-0004 | n/a |
| A13 | Colorado United Colorado United - 2008B Red |
CS25-25CB16-0111 | Brady Clapsaddle |