| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Skyline Skyline 07B Blue |
CS62-62CB16-0100 | Raul Coronado |
| A2 | Colorado EDGE Soccer Club Colorado EDGE Legends 07B |
CS54-54CB16-0088 | Willliam Reynolds |
| A3 | Chaffee County United SC Chaffee County United SC - BU16 |
CS12-12CB16-0006 | Aaron Dobson |
| A4 | Pride SC Pride SC United '07B |
CS51-51CB16-0101 | Mick Emmerson |
| A5 | Indios Denver SC Indios Nexaca BU16 Boys |
CS38-38CB16-0011 | n/a |
| A6 | Colorado United Colorado United - 2007B Blue |
CS25-25CB16-0113 | Andrew Coons |
| A7 | Colorado Rapids Colorado Rapids South 2007B Burgundy |
CS23-23CB16-0423 | Noah Aboneh |
| A8 | Colorado Rapids Colorado Rapids Central 2007B Burgundy |
CS23-23CB16-0443 | Danilson Pinto |
| A9 | Roaring Fork United Roaring Fork United '07B Eagles |
CS57-57CB16-0070 | John Spiess |
| A10 | Colorado Rapids Colorado Rapids Castle Rock 2007B Burgundy |
CS23-23CB16-0448 | Zach Ashland |
| A11 | Colorado Rush Colorado Rush - 2007B Azul |
CS24-24CB16-0067 | Justin Trujillo |
| A12 | Bright Stars of Colorado Soccer Academy Bright Stars Soccer Academy - BU16 |
CS07-07CB16-0009 | Enrique Guerrero Sr |