| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Arsenal Colorado Arsenal Colorado 05/04B Gold |
CS01-01CB19-0071 | Austin Scalf |
| A2 | Colorado Rush Colorado Rush - 2005B Academy |
CS24-24CB18-0066 | Ibro Arnautovic |
| A3 | Pride SC Pride SC Copa '05B |
CS51-51CB18-0092 | Sawyer Lincoln |
| A4 | Denver Kickers Sport Club DENVER KICKERS U18B (2005) RED |
CS28-28CB18-0028 | Nolan Helmus |
| A5 | Northern Colorado Rush NorCoRush 04B Blue (Smario) |
CS45-45CB19-0017 | John Smario |
| A6 | Pride SC Pride SC Copa '04B |
CS51-51CB19-0091 | Meghan Krause |
| A7 | Denver Kickers Sport Club DENVER KICKERS U19B (2004) RED |
CS28-28CB19-0029 | James Smith |
| A8 | Steamboat Springs YSA Steamboat Soccer Club - BU19 |
CS63-63CB19-0015 | Hobey Early |
| A9 | Colorado United Colorado United - 05/06B Blue |
CS25-25CB18-0115 | John Senkosky |
| A10 | Real Colorado Real CO 04.05 Boys Olympico |
CS53-53CB19-0230 | Steven Carels |
| A11 | FC Brighton FC Brighton 04B Red |
CS31-31CB19-0014 | Owen Darby |